Study on Diversity of Culturable Bacteria in Maize Field Soil under Pesticide Stress

The effects of pesticide application on the diversity culturable bacteria in soil corn farmland Sanjiang Plain were studied, and capable degrading acetochloramine samples screened. In black where “ethyldiazine 86” was applied, experimental group control set, two groups cultured screened by coating plate method. 16S rRNA gene sequences morphological characteristics analyzed to determine species bacteria. Selective medium designed screen 19481 strains experiment, including 7854 11627 group. A total 65 screened, purified preserved, 16 phosphorus solubilizing bacteria, 9 nitrogen fixing 4 community structure mainly composed Pseudomonas, Pantoea Bacillus, dominant bacterium Pseudomonas. four pesticide-degrading all Pseudomonas migulae. Long-term pesticides can reduce number culturable-bacteria soil, richness is also decreased. This study provides a theoretical basis for understanding bacterial residue protection sustainable utilization soil.